ARG23196
anti-Collagen III antibody
anti-Collagen III antibody for ELISA,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Collagen III. This product is specific for human collagen type III. It has shown the following cross-reactivities. Human collagen type I<15% Human collagen type IV<5% Human collagen type II and V<1% Human plasma proteins do not interfere with binding of the antibody to collagen. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-Fr, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Collagen III |
| Antigen Species | Human |
| Immunogen | Collagen III from Human placenta. |
| Conjugation | Un-conjugated |
| Alternate Names | EDS4A; Collagen alpha-1(III) chain |
Application Instructions
| Pre Adsorbed | This product has been cross-absorbed with immobilised human collagen types I, IV and V and with human blood plasma proteins. | ||||||||
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| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Serum |
| Buffer | PBS / BBS, 1% Dextran and 1% Mannitol. |
| Stabilizer | 1% Dextran and 1% Mannitol |
| Concentration | 0.1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | COL3A1 |
| Gene Full Name | collagen, type III, alpha 1 |
| Background | This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008] |
| Function | Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12. [UniProt] |
| Calculated MW | 139 kDa |
| PTM | Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. [UniProt] |
