ARG24008

anti-Collagen II antibody

anti-Collagen II antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Chicken

Overview

Product Description Rabbit Polyclonal antibody recognizes Collagen II
Tested Reactivity Chk
Tested Application ELISA, ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Collagen II
Antigen Species Chicken
Immunogen Native type II collagen extracted from Chicken cartilage.
Conjugation Un-conjugated
Alternate Names AOM; ANFH; SEDC; STL1; COL11A3; Collagen alpha-1(II) chain; Alpha-1 type II collagen)

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:2000
ICC/IF1:40
IHC-P1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 395069 Chicken COL2A1

Gene Symbol COL2A1
Gene Full Name collagen, type II, alpha 1
Background This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
Function Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Calculated MW 142 kDa
PTM Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.

The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG24008 anti-Collagen II antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Chicken cartilage tissue stained with ARG24008 anti-Collagen II antibody.