ARG41086
anti-Coilin antibody
anti-Coilin antibody for Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes Coilin |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Rat |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Coilin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human Coilin. (RLVRDNDCLRVKLE, NP_004636.1) |
| Conjugation | Un-conjugated |
| Alternate Names | Coilin; p80-coilin; CLN80 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COIL |
| Gene Full Name | coilin |
| Background | The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq, Jul 2008] |
| Function | Component of nuclear coiled bodies, also known as Cajal bodies or CBs, which are involved in the modification and assembly of nucleoplasmic snRNPs. [UniProt] |
| Cellular Localization | Nucleus. Nucleus, Cajal body. [UniProt] |
| Calculated MW | 63 kDa |
| PTM | Symmetrical dimethylation of arginine residues within the RG repeat region enhances affinity for SMN, and thus localization of SMN complexes to CBs. Phosphorylation during mitosis is associated with disassembly of CBs. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG41086 anti-Coilin antibody WB image
Western blot: 35 µg of MCF7 nuclear lysate (in RIPA buffer) stained with ARG41086 anti-Coilin antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
