ARG43118
anti-Claudin 5 antibody
anti-Claudin 5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Claudin 5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Pig |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Claudin 5 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human Claudin 5. |
| Conjugation | Un-conjugated |
| Alternate Names | Claudin-5; TMVCF; CPETRL1; BEC1; TMDVCF; Transmembrane protein deleted in VCFS; AWAL |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | A549 | ||||||||
| Observed Size | 23 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CLDN5 |
| Gene Full Name | claudin 5 |
| Background | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018] |
| Function | Plays a major role in tight junction-specific obliteration of the intercellular space. [UniProt] |
| Cellular Localization | Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 23 kDa |
Images (5) Click the Picture to Zoom In
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ARG43118 anti-Claudin 5 antibody ICC/IF image
Immunofluorescence: bEnd.3 cells were fixed with 4% paraformaldehyde for 15 min and permeabilized by 0.1% Triton X-100 for 15 min. Cells were stained with ARG43118 anti-Claudin 5 antibody (red) at 1:200 dilution. DAPI (blue) for nuclear staining.
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ARG43118 anti-Claudin 5 antibody IHC-P image
Immunohistochemistry: Formaldehyde-fixed and paraffin-embedded Human normal tissues adjacent to gastric cancer. Antigen Retrieval: Heat mediation was performed in Citrate buffer. The tissue was blocked and stained with ARG43118 anti-Claudin 5 antibody at 1:100 dilution, overnight at 4°C.
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ARG43118 anti-Claudin 5 antibody WB image
Western blot: A549 cell lysate stained with ARG43118 anti-Claudin 5 antibody.
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ARG43118 anti-Claudin 5 antibody IHC-P image
Immunohistochemistry: Formaldehyde-fixed and paraffin-embedded Mouse stomach tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer. The tissue was blocked and stained with ARG43118 anti-Claudin 5 antibody at 1:100 dilution, overnight at 4°C.
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ARG43118 anti-Claudin 5 antibody IHC-P image
Immunohistochemistry: Formaldehyde-fixed and paraffin-embedded Rat heart tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer. The tissue was blocked and stained with ARG43118 anti-Claudin 5 antibody at 1:100 dilution, overnight at 4°C.
