ARG66485
anti-Claudin 18 antibody
anti-Claudin 18 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes Claudin 18 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Target Name | Claudin 18 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human Claudin 18. |
| Conjugation | Un-conjugated |
| Alternate Names | Claudin-18; SFTPJ; SFTA5 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Citrate buffer (pH 6.0) was used. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CLDN18 |
| Gene Full Name | claudin 18 |
| Background | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010] |
| Function | Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. [UniProt] |
| Cellular Localization | Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 28 kDa |
Images (2) Click the Picture to Zoom In
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ARG66485 anti-Claudin 18 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG66485 anti-Claudin 18 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citric acid buffer (pH 6.0) was used.
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ARG66485 anti-Claudin 18 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach (high magnification) stained with ARG66485 anti-Claudin 18 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citrate buffer (pH 6.0) was used.
