ARG52873

anti-Claudin 10 antibody

anti-Claudin 10 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Signaling Transduction antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes Claudin 10
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Claudin 10
Antigen Species Human
Immunogen Synthetic peptide from C-terminus of human claudin-10 protein.
Conjugation Un-conjugated
Alternate Names OSP-L; CPETRL3; Claudin-10; OSP-like protein

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50
WB1:500 - 1:1000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Brain, Skeletal Muscle, Ovarian Carcinoma

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9071 Human CLDN10

Swiss-port # P78369 Human Claudin-10

Background The claudin superfamily consists of many structurally related proteins in humans. These proteins, which include claudin 1 through 18, are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Claudins, which consist of four trans-membrane domains and two extracellular loops, make up tight junction strands.
Cellular Localization cytoplasm
Research Area Signaling Transduction antibody
Calculated MW 24 kDa

Images (1) Click the Picture to Zoom In

  • ARG52873 anti-Claudin 10 antibody IHC-P image

    Immunohistochemistry: Human Skeletal Muscle stained with ARG52873 anti-Claudin 10 antibody.

Customer's Feedback

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Review for anti-Claudin 10 antibody

Application:WB

Sample:Mouse kidney

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC