ARG64743
anti-Clathrin light chain antibody
anti-Clathrin light chain antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Clathrin light chain |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize all reported isoforms of light chains a and b. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Clathrin light chain |
| Antigen Species | Human |
| Immunogen | CDFNPKSSKQAKD |
| Conjugation | Un-conjugated |
| Alternate Names | Clathrin light chain A; Lca; LCA |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CLTA |
| Gene Full Name | clathrin, light chain A |
| Background | Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, May 2010] |
| Function | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. [UniProt] |
| Research Area | Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 27 kDa |
Images (2) Click the Picture to Zoom In
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ARG64743 anti-Clathrin light chain antibody WB image
Western blot: 35 µg of Human Frontal Cortex lysate stained with ARG64743 anti-Clathrin light chain antibody at 0.1 µg/ml dilution.
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ARG64743 anti-Clathrin light chain antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64743 anti-Clathrin light chain antibody at 2.5 µg/ml dilution followed by AP-staining.
