ARG63979
anti-Chromogranin A precursor antibody
anti-Chromogranin A precursor antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Chromogranin A precursor |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Chromogranin A precursor |
| Antigen Species | Human |
| Immunogen | C-QGAKERAHQQKKH |
| Conjugation | Un-conjugated |
| Alternate Names | Vasostatin II; CGA; Vasostatin I; Pituitary secretory protein I; SP-I; CgA; SL21; Chromogranin-A |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 51 kDa |
| PTM | Sulfated on tyrosine residues and/or contains sulfated glycans. O-glycosylated with core 1 or possibly core 8 glycans. Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584). Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725). |
Images (5) Click the Picture to Zoom In
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ARG63979 anti-Chromogranin A precursor antibody WB image
Western Blot: human kidney lysate (35 µg protein in RIPA buffer) stained with ARG63979 anti-Chromogranin A precursor antibody at 0.1 µg/ml dilution.
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ARG63979 anti-Chromogranin A precursor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63979 anti-Chromogranin A precursor antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63979 anti-Chromogranin A precursor antibody WB image
Western blot: 35 µg of Human brain lysate (A) and isoform 1+2 (B) (in RIPA buffer) stained with ARG63979 anti-Chromogranin A precursor antibody at 2 µg/ml dilution and incubated at RT for 1 hour.
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ARG63979 anti-Chromogranin A precursor antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A549 cells permeabilized with 0.5% Triton. Cells were stained with ARG63979 anti-Chromogranin A precursor antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG63979 anti-Chromogranin A precursor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63979 anti-Chromogranin A precursor antibody at 3.75 µg/ml dilution followed by AP-staining.
