ARG52865
anti-Chromogranin A antibody
anti-Chromogranin A antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Chromogranin A |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Chromogranin A |
| Antigen Species | Human |
| Immunogen | Recombinant protein encoding human chromogranin A. |
| Conjugation | Un-conjugated |
| Alternate Names | Vasostatin II; CGA; Vasostatin I; Pituitary secretory protein I; SP-I; CgA; SL21; Chromogranin-A |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Pancreas |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Chromogranin A (a protein of 439 amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is expressed in carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. |
| Cellular Localization | Cytoplasm |
| Research Area | Cancer antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 51 kDa |
| PTM | Sulfated on tyrosine residues and/or contains sulfated glycans. O-glycosylated with core 1 or possibly core 8 glycans. Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584). Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725). |
Images (1) Click the Picture to Zoom In
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ARG52865 anti-Chromogranin A antibody IHC-P image
Immunohistochemistry: Human Pancreas stained with ARG52865 anti-Chromogranin A antibody
Specific References
Shigella flexneri Targets Human Colonic Goblet Cells by O Antigen Binding to Sialyl-Tn and Tn Antigens via Glycan-Glycan Interactions.
ICC/IF / Human
