ARG55538
anti-ChAT antibody
anti-ChAT antibody for ICC/IF,Western blot and Human,Mouse
Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ChAT |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ChAT |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 98-128 (N-terminus) of Human ChAT. |
| Conjugation | Un-conjugated |
| Alternate Names | CMS1A; Choline acetylase; CHOACTase; CHOACTASE; EC 2.3.1.6; CMS6; Choline O-acetyltransferase; ChAT; CMS1A2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | NCI-H460 |
Properties
| Form | Liquid |
|---|---|
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CHAT |
| Gene Full Name | choline O-acetyltransferase |
| Background | This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010] |
| Function | Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. [UniProt] |
| Research Area | Neuroscience antibody |
| Calculated MW | 83 kDa |
Images (2) Click the Picture to Zoom In
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ARG55538 anti-ChAT antibody ICC/IF image
Immunofluorescence: NCI-H460 cells stained with ARG55538 anti-ChAT antibody (green). DAPI (blue) for nuclear staining.
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ARG55538 anti-ChAT antibody WB image
Western blot: 35 µg of NCI-H460 cell lysate stained with ARG55538 anti-ChAT antibody.
