ARG52242
anti-ChAT antibody
anti-ChAT antibody for IHC-Frozen sections,Western blot and Human,Mouse,Rat,Chicken,Guinea pig,Primates
Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ChAT |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Chk, Gpig, NHuPrm |
| Tested Application | IHC-Fr, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ChAT |
| Antigen Species | Human |
| Immunogen | Native choline acetyltransferase purifed from human placenta |
| Conjugation | Un-conjugated |
| Alternate Names | CMS1A; Choline acetylase; CHOACTase; CHOACTASE; EC 2.3.1.6; CMS6; Choline O-acetyltransferase; ChAT; CMS1A2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | Specific for the ~ 70k choline acetyltransferase protein * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CHAT |
| Gene Full Name | choline O-acetyltransferase |
| Background | Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999). |
| Research Area | Neuroscience antibody |
| Calculated MW | 83 kDa |
Images (1) Click the Picture to Zoom In
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ARG52242 anti-ChAT antibody WB image
Western blot: rat brain lysate stained with ARG52242 anti-ChAT antibody showing specific immunolabeling of the ~70k ChAT.
