ARG64908

anti-Caveolin 3 antibody

anti-Caveolin 3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Caveolin 3
Tested Reactivity Hu, Ms, Rat, Pig
Predict Reactivity Cow, Dog
Tested Application IHC-P, WB
Specificity Reported variants represent identical protein (NP_001225.1; NP_203123.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Caveolin 3
Antigen Species Human
Immunogen EHTDLEAQIVKDIH-C
Conjugation Un-conjugated
Alternate Names VIP-21; VIP21; LQT9; Caveolin-3; LGMD1C; M-caveolin

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12391 Mouse CAV3

GeneID: 29161 Rat CAV3

GeneID: 641358 Pig CAV3

Background This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 17 kDa
PTM Sumoylation with SUMO3 by PIAS4 may reduce agonist-induced internalization and desensitization of adrenergic receptor ABRD2.

Images (4) Click the Picture to Zoom In

  • ARG64908 anti-Caveolin 3 antibody WB image

    Western blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG64908 anti-Caveolin 3 antibody at 0.3 µg/ml dilution.

  • ARG64908 anti-Caveolin 3 antibody WB image

    Western blot: Mouse (A) and Rat (B) Heart lysates (35 µg protein in RIPA buffer) stained with ARG64908 anti-Caveolin 3 antibody at 0.3 µg/ml dilution.

  • ARG64908 anti-Caveolin 3 antibody WB image

    Western blot: Pig Skeletal Muscle (A) and Heart (B) lysates (35 µg protein in RIPA buffer) stained with ARG64908 anti-Caveolin 3 antibody at 0.1 µg/ml dilution.

  • ARG64908 anti-Caveolin 3 antibody IHC-P image

    Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64908 anti-Caveolin 3 antibody at 3.8 µg/ml dilution followed by AP-staining.