ARG64908
anti-Caveolin 3 antibody
anti-Caveolin 3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Caveolin 3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P, WB |
| Specificity | Reported variants represent identical protein (NP_001225.1; NP_203123.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Caveolin 3 |
| Antigen Species | Human |
| Immunogen | EHTDLEAQIVKDIH-C |
| Conjugation | Un-conjugated |
| Alternate Names | VIP-21; VIP21; LQT9; Caveolin-3; LGMD1C; M-caveolin |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 17 kDa |
| PTM | Sumoylation with SUMO3 by PIAS4 may reduce agonist-induced internalization and desensitization of adrenergic receptor ABRD2. |
Images (4) Click the Picture to Zoom In
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ARG64908 anti-Caveolin 3 antibody WB image
Western blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG64908 anti-Caveolin 3 antibody at 0.3 µg/ml dilution.
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ARG64908 anti-Caveolin 3 antibody WB image
Western blot: Mouse (A) and Rat (B) Heart lysates (35 µg protein in RIPA buffer) stained with ARG64908 anti-Caveolin 3 antibody at 0.3 µg/ml dilution.
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ARG64908 anti-Caveolin 3 antibody WB image
Western blot: Pig Skeletal Muscle (A) and Heart (B) lysates (35 µg protein in RIPA buffer) stained with ARG64908 anti-Caveolin 3 antibody at 0.1 µg/ml dilution.
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ARG64908 anti-Caveolin 3 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64908 anti-Caveolin 3 antibody at 3.8 µg/ml dilution followed by AP-staining.
