ARG63969

anti-Catalase antibody

anti-Catalase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Peroxisome Marker antibody

Overview

Product Description Goat Polyclonal antibody recognizes Catalase
Tested Reactivity Hu, Ms
Predict Reactivity Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Catalase
Antigen Species Human
Immunogen C-DKYNAEKPKNAIHT
Conjugation Un-conjugated
Alternate Names Catalase; EC 1.11.1.6

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay - dependent
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12359 Mouse CAT

GeneID: 847 Human CAT

Swiss-port # P04040 Human Catalase

Swiss-port # P24270 Mouse Catalase

Background This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]
Highlight Related Antibody Duos and Panels:
ARG30310 Endosome, Lysosome, Peroxisome Marker Antibody Panel (Catalase, Caveolin1, Clathrin heavy chain, LAMP1)
Related products:
Catalase antibodies; Catalase ELISA Kits; Catalase Duos / Panels; Anti-Goat IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Peroxisome Marker antibody
Calculated MW 60 kDa
PTM The N-terminus is blocked.

Images (3) Click the Picture to Zoom In

  • ARG63969 anti-Catalase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63969 anti-Catalase antibody at 2 µg/ml dilution followed by AP-staining.

  • ARG63969 anti-Catalase antibody IHC-P image

    Immunohistochemistry: Paraffin embedded Human Liver. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63969 anti-Catalase antibody at 2 µg/ml dilution followed by AP-staining.

  • ARG63969 anti-Catalase antibody WB image

    Western blot: 35 µg of Human kidney (A), Human liver (B), Mouse kidney (C) and Mouse liver (D) lysates (in RIPA buffer) stained with ARG63969 anti-Catalase antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.