ARG42743
anti-Catalase antibody
anti-Catalase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Catalase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Catalase |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to E344-L527 of Human Catalase. |
| Conjugation | Un-conjugated |
| Alternate Names | Catalase; EC 1.11.1.6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | ~ 57 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CAT |
| Gene Full Name | catalase |
| Background | This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009] |
| Function | Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. [UniProt] |
| Cellular Localization | Peroxisome. [UniProt] |
| Calculated MW | 60 kDa |
| PTM | The N-terminus is blocked. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG42743 anti-Catalase antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG42743 anti-Catalase antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42743 anti-Catalase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissue stained with ARG42743 anti-Catalase antibody at 1 µg/ml dilution.
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ARG42743 anti-Catalase antibody WB image
Western blot: Rat skeletal muscle, Mouse skeletal muscle and A549 whole cell lysates stained with ARG42743 anti-Catalase antibody at 0.5 µg/ml dilution.
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ARG42743 anti-Catalase antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG42743 anti-Catalase antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG42743 anti-Catalase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer tissue stained with ARG42743 anti-Catalase antibody at 1 µg/ml dilution.
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ARG42743 anti-Catalase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver tissue stained with ARG42743 anti-Catalase antibody at 1 µg/ml dilution.
