ARG66686
anti-Caspase 5 (cleaved p10) antibody
anti-Caspase 5 (cleaved p10) antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Caspase 5 (cleaved p10) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | The antibody detects fragment of activated Caspase 5 protein (cleaved p10) resulting from cleavage adjacent to Ser331. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Caspase 5 (cleaved p10) |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 290-370 of Human Caspase 5 (cleaved p10). |
| Conjugation | Un-conjugated |
| Alternate Names | Protease ICH-3; Caspase-5; Protease TY; CASP-5; EC 3.4.22.58; ICE; ICEREL-III; ICH-3; ICE(rel)III; rel |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | 293 | ||||
| Observed Size | ~ 10 kDa (cleaved) |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CASP5 |
| Gene Full Name | caspase 5, apoptosis-related cysteine peptidase |
| Background | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010] |
| Function | Mediator of programmed cell death (apoptosis). [UniProt] |
| Calculated MW | 50 kDa (uncleaved) |
| PTM | The two subunits are derived from the precursor sequence by an autocatalytic mechanism. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66686 anti-Caspase 5 (cleaved p10) antibody WB image
Western blot: 293 cells treated with etoposide (25 µM for 1 hour). Cell lysates were stained with ARG66686 anti-Caspase 5 (cleaved p10) antibody. The lane on the right is blocked with the synthetic peptide.
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ARG66686 anti-Caspase 5 (cleaved p10) antibody WB image
Western blot: 293 cell lysate stained with ARG66686 anti-Caspase 5 (cleaved p10) antibody at 1:1000 dilution.
