ARG63702
anti-Caspase 4 antibody
anti-Caspase 4 antibody for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Caspase 4 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Cat, Cow, Dog |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP_150649.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Caspase 4 |
| Antigen Species | Human |
| Immunogen | CKERAEEIYPIKERN |
| Conjugation | Un-conjugated |
| Alternate Names | Mih1/TX; Protease ICH-2; Caspase-4; EC 3.4.22.57; TX; CASP-4; Protease TX; ICE; ICH-2; ICE(rel)II; rel; ICEREL-II |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody |
| Calculated MW | 43 kDa |
| PTM | In response to activation signals, including endoplasmic reticulum stress or treatment with amyloid beta A4 protein fragments (such as beta-amyloid protein 40), undergoes autoproteolytic cleavage. |
Images (1) Click the Picture to Zoom In
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ARG63702 anti-Caspase 4 antibody WB image
Western blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG63702 anti-Caspase 4 antibody at 0.3 µg/ml dilution.
