ARG63958
anti-Casein Kinase 1 delta antibody
anti-Casein Kinase 1 delta antibody for ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Casein Kinase 1 delta |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_001884.2 and NP_620693.1) |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Casein Kinase 1 delta |
| Antigen Species | Human |
| Immunogen | C-DREERLRHSRNPATR |
| Conjugation | Un-conjugated |
| Alternate Names | ASPS; EC 2.7.11.26; CKIdelta; CKId; HCKID; Casein kinase I isoform delta; Tau-protein kinase CSNK1D; EC 2.7.11.1; CKI-delta; FASPS2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 47 kDa |
| PTM | Autophosphorylated on serine and threonine residues; this autophosphorylation represses activity. Reactivated by phosphatase-mediated dephosphorylation. May be dephosphorylated by PP1. |
Images (2) Click the Picture to Zoom In
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ARG63958 anti-Casein Kinase 1 delta antibody WB image
Western blot: 293 cell lysate (35 µg protein in RIPA buffer) stained with ARG63958 anti-Casein Kinase 1 delta antibody at 0.3 µg/ml dilution.
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ARG63958 anti-Casein Kinase 1 delta antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U251 cells permeabilized with 0.15% Triton. Cells were stained with ARG63958 anti-Casein Kinase 1 delta antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
