ARG65648
anti-Cardiac Troponin T antibody
anti-Cardiac Troponin T antibody for Western blot and Human,Mouse,Rat,Pig
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Cardiac Troponin T |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Cat, Cow, Dog |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize all reported isoforms (NP_000355.2; NP_001001430.1; NP_001001431.1; NP_001001432.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Target Name | Cardiac Troponin T |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Human Cardiac Troponin T (C-RNRINDNQKVSKT) |
| Conjugation | Un-conjugated |
| Alternate Names | RCM3; CMPD2; LVNC6; TnTC; TnTc; CMH2; cTnT; Cardiac muscle troponin T; CMD1D; Troponin T, cardiac muscle |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TNNT2 |
| Gene Full Name | troponin T type 2 (cardiac) |
| Background | The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008] |
| Function | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 35.6 kDa (NP_000355.2) |
| PTM | Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity. |
Images (2) Click the Picture to Zoom In
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ARG65648 anti-Cardiac Troponin T antibody WB image
Western blot: 35 μg of Rat Heart lysate stained with ARG65648 anti-Cardiac Troponin T antibody at 0.03 μg/ml dilution (1 hour incubation).
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ARG65648 anti-Cardiac Troponin T antibody WB image
Western blot: 35 μg of Pig Heart lysate stained with ARG65648 anti-Cardiac Troponin T antibody at 0.01 μg/ml dilution (1 hour incubation).
