ARG55123
anti-Calreticulin antibody
anti-Calreticulin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Calreticulin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Calreticulin |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human Calreticulin (Swiss: P27797) |
| Conjugation | Un-conjugated |
| Alternate Names | CALR; Calreticulin; Calregulin; CC1qR; SSA; CRT; RO; Sicca Syndrome Antigen A (Autoantigen Ro; Calreticulin); Endoplasmic Reticulum Resident Protein 60; FLJ26680; CALR1; CRP55; ERp60; HACBP; Grp60; Epididymis Secretory Sperm Binding Protein Li 99n; Autoantigen Ro; HEL-S-99n; CRTC |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CALR |
| Gene Full Name | calreticulin |
| Background | Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type. |
| Function | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. |
| Cellular Localization | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Extracellular matrix, Lysosome, Sarcoplasmic reticulum, Secreted |
| Highlight | Related Antibody Duos and Panels: ARG30316 ER Marker Antibody Duo Related products: Calreticulin antibodies; Calreticulin Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody; ER Marker antibody; Endoplasmic reticulum antibody |
| Calculated MW | 48 kDa |
| PTM | Acetylation, Disulfide bond, Glycoprotein, Hydroxylation |
Images (3) Click the Picture to Zoom In
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ARG55123 anti-Calreticulin antibody WB image
Western blot: 30 µg of HeLa, HT29, A549, MCF-7, HepG2, Jurkat, K562, Mouse ovary and Rat ovary lysates stained with ARG55123 anti-Calreticulin antibody at 1:500 dilution.
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ARG55123 anti-Calreticulin antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55123 anti-Calreticulin antibody (red) at 1:20 dilution.
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
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ARG55123 anti-Calreticulin antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55123 anti-Calreticulin antibody at 1:20 dilution. Left: primary antibody (red). Middle: DAPI (blue). Right: Merge.
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
Customer's Feedback
Excellent
Review for anti-Calreticulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:20
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
Review for anti-Calreticulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:20
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：TRITC
Excellent
Review for anti-Calreticulin antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：Methanol / Acetone 1:1 ratio
Fixation Time：20 min
Fixation Temperature：-20 ºC
Antigen Retrieval Buffer：N/A
Antigen Retrieval Time：N/A
Antigen Retrieval Temperature：N/A ºC
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：1 hour
Primary Antibody Incubation Temperature：RT ºC
Excellent
Review for anti-Calreticulin antibody
Application：WB
Sample：NIH-3T3
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Down-regulation of calreticulin promotes apoptosis in hepatic stellate cells.
WB / Human
