ARG54629
anti-Calpastatin antibody [CSL1 5]
anti-Calpastatin antibody [CSL1 5] for ELISA,Immunohistochemistry,Western blot and Human
Cell Biology and Cellular Response antibody; Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [CSL1 5] recognizes Calpastatin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC, WB |
| Specificity | This antibody specifically recognizes an epitope within domain III of human calpastatin. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CSL1 5 |
| Isotype | IgG1 |
| Target Name | Calpastatin |
| Antigen Species | Human |
| Immunogen | Recombinant human muscle-type calpastatin. |
| Conjugation | Un-conjugated |
| Alternate Names | Calpastatin; PLACK; Calpain inhibitor; Sperm BS-17 component; BS-17 |
Application Instructions
| Application Note | ELISA: 1 - 10 ug/ml with immobilized antigen. Western blot: 1 - 10 ug/ml, denaturing and non-denaturing conditions. Immunohistochemistry: 1 - 10 ug/ml, frozen tissue sections. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Buffer | 10 mM PBS (pH 7.4) and 1% BSA |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CAST |
| Gene Full Name | calpastatin |
| Background | The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010] |
| Function | Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Neuroscience antibody |
| Calculated MW | 77 kDa |
| PTM | The N-terminus is blocked. |
