ARG62379
anti-Caldesmon antibody [h-CALD]
anti-Caldesmon antibody [h-CALD] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [h-CALD] recognizes Caldesmon |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | Specific to human caldesmon (150 kDa protein) |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | h-CALD |
| Isotype | IgG1 |
| Target Name | Caldesmon |
| Antigen Species | Human |
| Immunogen | BALB / C mice were injected with crude human uterus extract. |
| Conjugation | Un-conjugated |
| Alternate Names | CDM; HCAD; Caldesmon; NAG22; L-CAD; LCAD; H-CAD |
Application Instructions
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| Positive Control | Uterus |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CALD1 |
| Gene Full Name | caldesmon 1 |
| Background | This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| Function | Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity). [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 93 kDa |
| PTM | In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity). |
Clone References
Resident phenotypically modulated vascular smooth muscle cells in healthy human arteries.
ICC/IF / Human
Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways?
ICC/IF / Human
Morphological and mechanical characteristics of the reconstructed rat abdominal wall following use of a wet electrospun biodegradable polyurethane elastomer scaffold.
IHC-FoFr / Rat
TGF-beta1 drives partial myofibroblastic differentiation in chondromyxoid fibroma of bone.
IHC / Human
