ARG40256
anti-Calbindin antibody
anti-Calbindin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Calbindin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Calbindin |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-261 of Human Calbindin (NP_004920.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Vitamin D-dependent calcium-binding protein, avian-type; Calbindin; CALB; Calbindin D28; D-28K |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | A549 and Mouse brain | ||||||
| Observed Size | ~ 30 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CALB1 |
| Gene Full Name | calbindin 1, 28kDa |
| Background | The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015] |
| Function | Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. [UniProt] |
| Calculated MW | 30 kDa |
Images (2) Click the Picture to Zoom In
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ARG40256 anti-Calbindin antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG40256 anti-Calbindin antibody at 1:1000 dilution.
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ARG40256 anti-Calbindin antibody WB image
Western blot: 25 µg of Mouse brain lysate stained with ARG40256 anti-Calbindin antibody at 1:1000 dilution.
Specific References
Targeting of Calbindin 1 rescues erythropoiesis in a human model of Diamond Blackfan anemia
WB / Human
