ARG52238
anti-CaMKII phospho (Thr286) antibody
anti-CaMKII phospho (Thr286) antibody for Western blot and Rat
Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CaMKII phospho (Thr286) |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Xenopus laevis |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CaMKII |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | CAMKA; CaMK-II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; EC 2.7.11.17 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | Specific for the ~50k α-CaM Kinase II and the ~60k β-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the λ-phosphatase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11275 Rat Calcium/calmodulin-dependent protein kinase type II subunit alpha |
|---|---|
| Gene Symbol | CAMK2A/B |
| Gene Full Name | calcium/calmodulin-dependent protein kinase II alpha |
| Background | Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002). |
| Research Area | Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 54 kDa |
Images (1) Click the Picture to Zoom In
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ARG52238 anti-CaMKII phospho (Thr286) antibody WB image
Western blot: Rat cortex lysate showing phospho-specific immunolabeling of the ~50 kDa alpha-subunit and ~60 kDa beta-subunit of the CaM Kinase II protein phosphorylated at Thr 286 stained with ARG52238 anti-CaMKII phospho (Thr286) antibody.
