ARG55222
anti-CYC1 antibody
anti-CYC1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CYC1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CYC1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 142-176 (Center) of Human CYC1. |
| Conjugation | Un-conjugated |
| Alternate Names | Cytochrome c1, heme protein, mitochondrial; UQCR4; Cytochrome b-c1 complex subunit 4; Complex III subunit 4; Complex III subunit IV; Cytochrome c-1; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; MC3DN6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08574 Human Cytochrome c1, heme protein, mitochondrial Swiss-port # Q9D0M3 Mouse Cytochrome c1, heme protein, mitochondrial |
|---|---|
| Gene Symbol | CYC1 |
| Gene Full Name | cytochrome c-1 |
| Background | This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c. Mutations in this gene may cause mitochondrial complex III deficiency, nuclear type 6. [provided by RefSeq, Dec 2013] |
| Function | This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein; Intermembrane side |
| Research Area | Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 35 kDa |
| PTM | Binds 1 heme group per subunit. |
Images (3) Click the Picture to Zoom In
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ARG55222 anti-CYC1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55222 anti-CYC1 antibody (green) at 1:25 dilution. DAPI (blue) for nuclear staining. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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ARG55222 anti-CYC1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach tissue stained with ARG55222 anti-CYC1 antibody at 1:25 dilution.
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ARG55222 anti-CYC1 antibody WB image
Western blot: 35 µg of HeLa cell lysate stained with ARG55222 anti-CYC1 antibody at 1:1000 dilution.
Customer's Feedback
Excellent
Review for anti-CYC1 antibody
Application：WB
Sample：HeLa and MCF7
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
