ARG56670

anti-CXCL7 / NAP2 antibody

anti-CXCL7 / NAP2 antibody for ELISA,Western blot and Human

Overview

Product Description Goat Polyclonal antibody recognizes CXCL7 / NAP2
Tested Reactivity Hu
Tested Application ELISA, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name CXCL7 / NAP2
Antigen Species Human
Immunogen E.coli derived Recombinant Human NAP-2 (CXCL7).
(AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD)
Conjugation Un-conjugated
Alternate Names CTAP3; Platelet basic protein; SCYB7; Macrophage-derived growth factor; THBGB; CTAPIII; C-X-C motif chemokine 7; Beta-TG; NAP-2; Small-inducible cytokine B7; TGB; THBGB1; CTAP-III; PBP; MDGF; TC2; Low-affinity platelet factor IV; Leukocyte-derived growth factor; TC1; 74; 73; 1-66; B-TG1; 1-63; CXCL7; TGB1; 1-81; LDGF; LA-PF4

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56780 as a detection antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5473 Human PPBP

Swiss-port # P02775 Human Platelet basic protein

Gene Symbol PPBP
Gene Full Name pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
Background The protein encoded by this gene is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. The protein also is an antimicrobial protein with bactericidal and antifungal activity. [provided by RefSeq, Nov 2014]
Function LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. [UniProt]
Calculated MW 14 kDa
PTM Proteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets.

Images (3) Click the Picture to Zoom In

  • ARG56670 anti-CXCL7 / NAP2 antibody WB image

    Western blot: 250 - 0.24 ng of Human NAP-2 stained with ARG56670 anti-CXCL7 / NAP2 antibody, under reducing conditions.

  • ARG56670 anti-CXCL7 / NAP2 antibody WB image

    Western blot: 250 - 0.24 ng of Human NAP-2 stained with ARG56670 anti-CXCL7 / NAP2 antibody, under non-reducing conditions.

  • ARG56670 anti-CXCL7 / NAP2 antibody standard curve image

    Sandwich ELISA: ARG56670 anti-CXCL7 / NAP2 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56780 anti-CXCL7 / NAP-2 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.