ARG56640
anti-CXCL4 / PF4 antibody
anti-CXCL4 / PF4 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CXCL4 / PF4 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CXCL4 / PF4 |
| Antigen Species | Human |
| Immunogen | E.coli derived Recombinant Human PF-4 (CXCL4). (EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES) |
| Conjugation | Un-conjugated |
| Alternate Names | Platelet factor 4; Iroplact; CXCL4; C-X-C motif chemokine 4; Oncostatin-A; SCYB4; PF-4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PF4 |
| Gene Full Name | platelet factor 4 |
| Background | This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014] |
| Function | Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. [UniProt] |
| Calculated MW | 11 kDa |
Images (6) Click the Picture to Zoom In
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ARG56640 anti-CXCL4 / PF4 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast malignant ductal adenocarcinoma. The recommended ARG56640 anti-CXCL4 / PF4 antibody concentration is 1.0 µg/mL - 2.0 µg/mL with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56640 anti-CXCL4 / PF4 antibody WB image
Western blot: 250 - 0.24 ng of Human PF-4 stained with ARG56640 anti-CXCL4 / PF4 antibody, under reducing conditions.
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ARG56640 anti-CXCL4 / PF4 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast malignant ductal adenocarcinoma. The recommended ARG56640 anti-CXCL4 / PF4 antibody concentration is 1.0 µg/mL - 2.0 µg/mL with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56640 anti-CXCL4 / PF4 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast malignant ductal adenocarcinoma. The recommended ARG56640 anti-CXCL4 / PF4 antibody concentration is 1.0 µg/mL - 2.0 µg/mL with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56640 anti-CXCL4 / PF4 antibody WB image
Western blot: 250 - 0.24 ng of Human PF-4 stained with ARG56640 anti-CXCL4 / PF4 antibody, under non-reducing conditions.
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ARG56640 anti-CXCL4 / PF4 antibody standard curve image
Sandwich ELISA: ARG56640 anti-CXCL4 / PF4 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56750 anti-CXCL4 / PF4 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
