ARG43362
anti-CXCL4 / PF4 antibody
anti-CXCL4 / PF4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CXCL4 / PF4 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CXCL4 / PF4 |
| Antigen Species | Rat |
| Immunogen | Recombinant protein corresponding to V30-S105 of Rat CXCL4 / PF4. |
| Conjugation | Un-conjugated |
| Alternate Names | Platelet factor 4; Iroplact; CXCL4; C-X-C motif chemokine 4; Oncostatin-A; SCYB4; PF-4 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Rat spleen | ||||||
| Observed Size | ~ 11 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PF4 |
| Gene Full Name | platelet factor 4 |
| Background | This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014] |
| Function | Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 11 kDa |
Images (3) Click the Picture to Zoom In
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ARG43362 anti-CXCL4 / PF4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43362 anti-CXCL4 / PF4 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43362 anti-CXCL4 / PF4 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat spleen lysate stained with ARG43362 anti-CXCL4 / PF4 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43362 anti-CXCL4 / PF4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43362 anti-CXCL4 / PF4 antibody at 1 µg/ml dilutioin, overnight at 4°C.
