ARG66032

anti-CX3CL1 antibody (Biotin)

anti-CX3CL1 antibody (Biotin) for ELISA,Western blot and Human

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes CX3CL1
Tested Reactivity Hu
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CX3CL1
Antigen Species Human
Immunogen E. coli derived recombinant Human CX3CL1.
(QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG)
Conjugation Biotin
Alternate Names neurotactin; CXC3; C3Xkine; NTN; NTT; C-X3-C motif chemokine 1; CXC3C; Neurotactin; ABCD-3; SCYD1; Fractalkine; Small-inducible cytokine D1; CX3C membrane-anchored chemokine; fractalkine

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66031 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6376 Human CX3CL1

Swiss-port # P78423 Human Fractalkine

Gene Symbol CX3CL1
Gene Full Name chemokine (C-X3-C motif) ligand 1
Function The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. [UniProt]
Calculated MW 42 kDa
PTM A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
O-glycosylated with core 1 or possibly core 8 glycans.

Images (2) Click the Picture to Zoom In

  • ARG66032 anti-CX3CL1 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human Fractalkine stained with ARG66032 anti-CX3CL1 antibody (Biotin), under non-reducing conditions.

  • ARG66032 anti-CX3CL1 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human Fractalkine stained with ARG66032 anti-CX3CL1 antibody (Biotin), under reducing conditions.