ARG42745
anti-CTHRC1 antibody
anti-CTHRC1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CTHRC1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CTHRC1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to S31-K243 of Human CTHRC1. |
| Conjugation | Un-conjugated |
| Alternate Names | Protein NMTC1; Collagen triple helix repeat-containing protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 26 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.01% Sodium azide and 4% Trehalose. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CTHRC1 |
| Gene Full Name | collagen triple helix repeat containing 1 |
| Background | This locus encodes a protein that may play a role in the cellular response to arterial injury through involvement in vascular remodeling. Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2012] |
| Function | May act as a negative regulator of collagen matrix deposition. [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
| Calculated MW | 26 kDa |
| PTM | N-glycosylated. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG42745 anti-CTHRC1 antibody ICC/IF image
Immunofluorescence: Hepa 1-6 cells stained with ARG42745 anti-CTHRC1 antibody (red) at 4 μg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42745 anti-CTHRC1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42745 anti-CTHRC1 antibody at 1 μg/ml dilution, overnight at 4°C.
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ARG42745 anti-CTHRC1 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat brain, Rat lung, Rat thymus, PC-12, Mouse thymus, Raw264.7, SP2/0 and Jurkat whole cell lysates stained with ARG42745 anti-CTHRC1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42745 anti-CTHRC1 antibody FACS image
Flow Cytometry: HepG2 cells were blocked with 10% normal goat serum and then stained with ARG42745 anti-CTHRC1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
