ARG64952
anti-CSX1 / NKX2-5 antibody
anti-CSX1 / NKX2-5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CSX1 / NKX2-5 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CSX1 / NKX2-5 |
| Antigen Species | Human |
| Immunogen | C-PRAYSDPDPAKDPR |
| Conjugation | Un-conjugated |
| Alternate Names | NKX2.5; NKX2E; Homeobox protein Nkx-2.5; CHNG5; NKX4-1; VSD3; CSX; CSX1; Homeobox protein NK-2 homolog E; HLHS2; Homeobox protein CSX; Cardiac-specific homeobox |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody |
| Calculated MW | 35 kDa |
Images (2) Click the Picture to Zoom In
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ARG64952 anti-CSX1 / NKX2-5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64952 anti-CSX1 / NKX2-5 antibody at 6 µg/ml dilution followed by AP-staining.
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ARG64952 anti-CSX1 / NKX2-5 antibody WB image
Western blot: 35 µg of Mouse heart lysate (in RIPA buffer) stained with ARG64952 anti-CSX1 / NKX2-5 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
