ARG42093
anti-CROT / COT antibody
anti-CROT / COT antibody for Western blot and Rat
Overview
| Product Description | Goat Polyclonal antibody recognizes CROT / COT |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CROT / COT |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide around the C-terminus of Rat CROT / COT. (RSCLETDAEKLVE) (NP_114193.1) |
| Conjugation | Un-conjugated |
| Alternate Names | Peroxisomal carnitine O-octanoyltransferase; COT; EC 2.3.1.137 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 75 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11466 Rat Peroxisomal carnitine O-octanoyltransferase |
|---|---|
| Gene Symbol | CROT |
| Gene Full Name | carnitine O-octanoyltransferase |
| Background | This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009] |
| Function | Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester. [UniProt] |
| Cellular Localization | Peroxisome. [UniProt] |
| Calculated MW | 70 kDa |
Images (1) Click the Picture to Zoom In
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ARG42093 anti-CROT / COT antibody WB image
Western blot: 35 µg of Rat kidney lysate (in RIPA buffer) stained with ARG42093 anti-CROT / COT antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
