ARG24101
anti-CRH / Corticotropin Releasing Factor antibody [4H9]
anti-CRH / Corticotropin Releasing Factor antibody [4H9] for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Mouse Monoclonal antibody [4H9] recognizes CRH / Corticotropin Releasing Factor |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, ICC/IF, IHC-Fr, IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4H9 |
| Isotype | IgG1 |
| Target Name | CRH / Corticotropin Releasing Factor |
| Antigen Species | Human |
| Immunogen | BSA conjugated full-length Human CRH / Corticotropin-Releasing Factor protein. |
| Epitope | The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRH / Corticotropin Releasing Factor (CRF)-binding protein, also inhibits the binding of this antibody to CRH / Corticotropin Releasing Factor (CRF). |
| Conjugation | Un-conjugated |
| Protein Full Name | Corticoliberin |
| Alternate Names | CRH; Corticoliberin; Corticotropin-releasing hormone; CRF; CRH1; Corticotropin-releasing factor |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) or enzyme digestion with 0.1% trypsin. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.1% Sodium azide. |
| Preservative | 0.1% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CRH |
| Gene Full Name | corticotropin releasing hormone |
| Background | Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010] |
| Function | This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 21.4 kDa |
| PTM | Amidation, Cleavage on pair of basic residues |
