ARG63660
anti-CREM antibody
anti-CREM antibody for Western blot and Human
Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes CREM |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise the following protein isoforms of human CREM protein: a (NP_853549.1); e (NP_874387.1); f (NP_874388.1); k (NP_874393.1); l (NP_874394.1); m (NP_877570.1); n (NP_877571.1); o (NP_877572.1); p (NP_877573.1); q (NP_878270.1); v (NP_898883.1). This antibody is not expected to cross-react with CREB protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CREM |
| Antigen Species | Human |
| Immunogen | LETLKDICSPKTDY |
| Conjugation | Un-conjugated |
| Alternate Names | cAMP-responsive element modulator; ICER; Inducible cAMP early repressor; CREM-2; hCREM-2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 35 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 37 kDa |
| PTM | Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome. Stimulated by phosphorylation. |
Images (1) Click the Picture to Zoom In
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ARG63660 anti-CREM antibody WB image
Western Blot: human heart lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63660 anti-CREM antibody at 1 µg/ml dilution.
