ARG56991

anti-CRABP1 antibody [1A1]

anti-CRABP1 antibody [1A1] for Flow cytometry,Western blot and Human,Mouse

Overview

Product Description Mouse Monoclonal antibody [1A1] recognizes CRABP1
Tested Reactivity Hu, Ms
Tested Application FACS, WB
Host Mouse
Clonality Monoclonal
Clone 1A1
Isotype IgG2b, kappa
Target Name CRABP1
Antigen Species Human
Immunogen Recombinant fragment around aa. 1-137 of Human CRABP1.
Conjugation Un-conjugated
Alternate Names CRABP; Cellular retinoic acid-binding protein I; CRABP-I; RBP5; Cellular retinoic acid-binding protein 1; CRABPI

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12903 Mouse CRABP1

GeneID: 1381 Human CRABP1

Swiss-port # P29762 Human Cellular retinoic acid-binding protein 1

Swiss-port # P62965 Mouse Cellular retinoic acid-binding protein 1

Gene Symbol CRABP1
Gene Full Name cellular retinoic acid binding protein 1
Background This gene encodes a specific binding protein for a vitamin A family member and is thought to play an important role in retinoic acid-mediated differentiation and proliferation processes. It is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid at specific sites within the nucleus, which may contribute to vitamin A-directed differentiation in epithelial tissue. [provided by RefSeq, Jul 2008]
Function Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors. [UniProt]
Calculated MW 16 kDa

Images (4) Click the Picture to Zoom In

  • ARG56991 anti-CRABP1 antibody [1A1] FACS image

    Flow Cytometry: Balb/3T3 cell line stained with ARG56991 anti-CRABP1 antibody [1A1] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).

  • ARG56991 anti-CRABP1 antibody [1A1] WB image

    Western blot: 30 µg of Mouse eye lysate stained with ARG56991 anti-CRABP1 antibody [1A1] at 1:500.

  • ARG56991 anti-CRABP1 antibody [1A1] WB image

    Western blot: 40 µg of MCF-7 cell lysate stained with ARG56991 anti-CRABP1 antibody [1A1] at 1:500.

  • ARG56991 anti-CRABP1 antibody [1A1] WB image

    Western blot: 20 µg of 1) 293T cell lysate, 2) CRABP1 transfected 293T cell lysate stained with ARG56991 anti-CRABP1 antibody [1A1] at 1:500.