ARG54965
anti-COX6B1 antibody
anti-COX6B1 antibody for ICC/IF,Western blot and Human
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes COX6B1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Mk |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | COX6B1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 8-22 (N-terminus) of Human COX6B1. |
| Conjugation | Un-conjugated |
| Alternate Names | COXVIb1; Cytochrome c oxidase subunit 6B1; Cytochrome c oxidase subunit VIb isoform 1; COXG; COX VIb-1; COX6B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COX6B1 |
| Gene Full Name | cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) |
| Background | Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIb. Mutations in this gene are associated with severe infantile encephalomyopathy. Three pseudogenes COX6BP-1, COX6BP-2 and COX6BP-3 have been found on chromosomes 7, 17 and 22q13.1-13.2, respectively. [provided by RefSeq, Jan 2010] |
| Function | Connects the two COX monomers into the physiological dimeric form. [UniProt] |
| Cellular Localization | Mitochondrion intermembrane space |
| Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 10 kDa |
Images (2) Click the Picture to Zoom In
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ARG54965 anti-COX6B1 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG54965 anti-COX6B1 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red). DAPI (blue) for nuclear staining.
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ARG54965 anti-COX6B1 antibody WB image
Western blot: 35 µg of HeLa cell lysate stained with ARG54965 anti-COX6B1 antibody at 1:1000 dilution.
