ARG41012
anti-COX5B antibody
anti-COX5B antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes COX5B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | COX5B |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 32-129 of Human COX5B (NP_001853.2). |
| Conjugation | Un-conjugated |
| Alternate Names | Cytochrome c oxidase polypeptide Vb; Cytochrome c oxidase subunit 5B, mitochondrial; COXVB |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 14 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P10606 Human Cytochrome c oxidase subunit 5B, mitochondrial Swiss-port # P12075 Rat Cytochrome c oxidase subunit 5B, mitochondrial |
|---|---|
| Gene Symbol | COX5B |
| Gene Full Name | cytochrome c oxidase subunit Vb |
| Background | Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008] |
| Function | This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane. [UniProt] |
| Calculated MW | 14 kDa |
Images (6) Click the Picture to Zoom In
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ARG41012 anti-COX5B antibody IHC-P image
Immunohistochemistry: Human colon stained with ARG41012 anti-COX5B antibody at 1:100 dilution.
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ARG41012 anti-COX5B antibody ICC/IF image
Immunofluorescence: U2OS stained with ARG41012 anti-COX5B antibody at 1:100 dilution.
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ARG41012 anti-COX5B antibody WB image
Western blot: HepG2 stained with ARG41012 anti-COX5B antibody at 1:1000 dilution.
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ARG41012 anti-COX5B antibody IHC-P image
Immunohistochemistry: Rat kidney stained with ARG41012 anti-COX5B antibody at 1:100 dilution.
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ARG41012 anti-COX5B antibody ICC/IF image
Immunofluorescence: C6 stained with ARG41012 anti-COX5B antibody at 1:100 dilution.
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ARG41012 anti-COX5B antibody ICC/IF image
Immunofluorescence: NIH-3T3 stained with ARG41012 anti-COX5B antibody at 1:100 dilution.
