ARG57153
anti-COX5A antibody [7A6]
anti-COX5A antibody [7A6] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [7A6] recognizes COX5A |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7A6 |
| Isotype | IgG1, kappa |
| Target Name | COX5A |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 42-150 of Human COX5A |
| Conjugation | Un-conjugated |
| Alternate Names | Cytochrome c oxidase subunit 5A, mitochondrial; VA; COX-VA; Cytochrome c oxidase polypeptide Va; COX |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P20674 Human Cytochrome c oxidase subunit 5A, mitochondrial |
|---|---|
| Gene Symbol | COX5A |
| Gene Full Name | cytochrome c oxidase subunit Va |
| Background | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq, Jul 2008] |
| Function | This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane. [UniProt] |
| Calculated MW | 17 kDa |
Images (2) Click the Picture to Zoom In
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ARG57153 anti-COX5A antibody [7A6] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57153 anti-COX5A antibody [7A6] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57153 anti-COX5A antibody [7A6] WB image
Western blot: 40 µg of 1) A431, and 2) HeLa cell lysates stained with ARG57153 anti-COX5A antibody [7A6] at 1:1000.
