ARG64285
anti-COX4 antibody
anti-COX4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes COX4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize COX4I1 (GeneID:1327; MW: 19.6kDa) & COX4I2 (GeneID:84701, MW:19.6kDa). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | COX4 |
| Antigen Species | Human |
| Immunogen | C-QGLASKWDYEKNE |
| Conjugation | Un-conjugated |
| Alternate Names | Cytochrome c oxidase polypeptide IV; COX4; COX4-1; COXIV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4I1 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P13073 Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
|---|---|
| Gene Symbol | COX4I1 |
| Gene Full Name | cytochrome c oxidase subunit IV isoform 1 |
| Background | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] |
| Function | COX4 protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. [UniProt] |
| Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody |
| Calculated MW | 20 kDa |
Images (3) Click the Picture to Zoom In
-
ARG64285 anti-COX4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid gland tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64285 anti-COX4 antibody at 2.5 µg/ml dilution, followed by AP-staining.
-
ARG64285 anti-COX4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64285 anti-COX4 antibody at 2.5 µg/ml dilution followed by AP-staining.
-
ARG64285 anti-COX4 antibody WB image
Western blot: 35 µg of Human muscle lysate stained with ARG64285 anti-COX4 antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
