ARG55304
anti-COX4 antibody
anti-COX4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes COX4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | COX4 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human COX IV (NP_001852.1) |
| Conjugation | Un-conjugated |
| Alternate Names | Cytochrome c oxidase polypeptide IV; COX4; COX4-1; COXIV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4I1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | Mouse kidney and HepG2 |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | COX4I1 |
| Gene Full Name | cytochrome c oxidase subunit IV isoform 1 |
| Background | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] |
| Function | COX4 protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. [UniProt] |
| Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody |
| Calculated MW | 20 kDa |
Images (2) Click the Picture to Zoom In
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ARG55304 anti-COX4 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55304 anti-COX4 antibody.
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ARG55304 anti-COX4 antibody WB image
Western blot: Mouse kidney and HepG2 cell lysates stained with ARG55304 anti-COX4 antibody.
Customer's Feedback
Excellent
Review for anti-COX4 antibody
Application：WB
Sample：A: Mouse brain, B: Rat brain, C: U87-MG
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
