ARG63193
anti-COX1 antibody
anti-COX1 antibody for ICC/IF,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes COX1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody is expected to recognise both human isoforms of this protein according to NP_000953.2 and NP_542158.1. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | COX1 |
| Antigen Species | Human |
| Immunogen | C-QDDGPAVERPSTEL |
| Conjugation | Un-conjugated |
| Alternate Names | Prostaglandin H2 synthase 1; COX-1; PHS1; Cyclooxygenase-1; PGHS1; COX3; COX1; PGG/HS; PTGHS; PES-1; EC 1.14.99.1; PHS 1; Prostaglandin-endoperoxide synthase 1; PGHS-1; PCOX1; PGH synthase 1; Prostaglandin G/H synthase 1 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis. Alternative splicing of this gene generates two transcript variants. The expression of these two transcripts is differentially regulated by relevant cytokines and growth factors. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 69 kDa |
Images (4) Click the Picture to Zoom In
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ARG63193 anti-COX1 antibody WB image
Western blot: HeLa lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63193 anti-COX1 antibody at 1 µg/ml dilution.
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ARG63193 anti-COX1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed NIH/3T3 cells permeabilized with 0.15% Triton. Cells were stained with ARG63193 anti-COX1 antibody (green) at 5 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 5 µg/ml dilution.
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ARG63193 anti-COX1 antibody WB image
Western blot: 35 µg of U937 cell lysate (in RIPA buffer) stained with ARG63193 anti-COX1 antibody at 2 µg/ml dilution and incubated at RT for 1 hour.
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ARG63193 anti-COX1 antibody WB image
Western blot: 35 µg of Mouse kidney (A) and skeletal muscle (B) lysates (in RIPA buffer) stained with ARG63193 anti-COX1 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
