ARG59767
anti-COPS5 / JAB1 antibody
anti-COPS5 / JAB1 antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes COPS5 / JAB1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | COPS5 / JAB1 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-334 of Human COPS5 (NP_006828.2). |
| Conjugation | Un-conjugated |
| Alternate Names | MOV-34; Jun activation domain-binding protein 1; EC 3.4.-.-; JAB1; Signalosome subunit 5; SGN5; COP9 signalosome complex subunit 5; CSN5 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | PC-12, NIH/3T3 and HeLa | ||||
| Observed Size | 37 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O35864 Mouse COP9 signalosome complex subunit 5 Swiss-port # Q92905 Human COP9 signalosome complex subunit 5 |
|---|---|
| Gene Symbol | COPS5 |
| Gene Full Name | COP9 signalosome subunit 5 |
| Background | The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. [provided by RefSeq, Jul 2008] |
| Function | Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2. [UniProt] |
| Cellular Localization | Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Note=Nuclear localization is diminished in the presence of IFIT3. [UniProt] |
| Calculated MW | 38 kDa |
Images (2) Click the Picture to Zoom In
-
ARG59767 anti-COPS5 / JAB1 antibody WB image
Western blot: 25 µg of PC-12, NIH/3T3 and HeLa cell lysates stained with ARG59767 anti-COPS5 / JAB1 antibody at 1:1000 dilution.
-
ARG59767 anti-COPS5 / JAB1 antibody WB image
Western blot: 25 µg of PC-12, NIH/3T3 and HeLa cell lysates stained with ARG59767 anti-COPS5 / JAB1 antibody at 1:1000 dilution through one-step method.
