ARG42481

anti-COPG2 antibody

anti-COPG2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes COPG2
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name COPG2
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 542-871 of Human COPG2 (NP_036265.3).
Conjugation Un-conjugated
Alternate Names Gamma-2-COP; Gamma-2-coat protein; gamma-2-COP; Coatomer subunit gamma-2; 2-COP

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat heart
Observed Size ~ 105 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 26958 Human COPG2

GeneID: 54160 Mouse COPG2

Swiss-port # Q9QXK3 Mouse Coatomer subunit gamma-2

Swiss-port # Q9UBF2 Human Coatomer subunit gamma-2

Gene Symbol COPG2
Gene Full Name coatomer protein complex, subunit gamma 2
Function The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). [UniProt]
Cellular Localization Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Tends to be more abundant in the trans-Golgi network compared to the cis-Golgi. [UniProt]
Calculated MW 98 kDa

Images (2) Click the Picture to Zoom In

  • ARG42481 anti-COPG2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue stained with ARG42481 anti-COPG2 antibody at 1:100 dilution.

  • ARG42481 anti-COPG2 antibody WB image

    Western blot: 25 µg of Rat heart lysate stained with ARG42481 anti-COPG2 antibody at 1:1000 dilution.