ARG64014
anti-COMT antibody
anti-COMT antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes COMT |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1) |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | COMT |
| Antigen Species | Human |
| Immunogen | C-QDIIPQLKKKYDVD |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.1.1.6; Catechol O-methyltransferase; HEL-S-98n |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: 1) Microwaved tissue section in Tris/EDTA buffer (pH 9.0). Or 2) Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008] |
| Research Area | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 30 kDa |
| PTM | The N-terminus is blocked. |
Images (4) Click the Picture to Zoom In
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ARG64014 anti-COMT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG64014 anti-COMT antibody at 4 µg/ml dilution followed by HRP-staining.
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ARG64014 anti-COMT antibody WB image
Western blot: 35 µg of Human testis lysate (in RIPA buffer) stained with ARG64014 anti-COMT antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG64014 anti-COMT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64014 anti-COMT antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64014 anti-COMT antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64014 anti-COMT antibody at 3.75 µg/ml dilution followed by AP-staining.
