ARG59753
anti-CNTN4 antibody
anti-CNTN4 antibody for Western blot and Mouse
Overview
| Product Description | Goat Polyclonal antibody recognizes CNTN4 |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Hu, Rat, Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CNTN4 |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide between aa. 160-172 of Mouse CNTN4. (NP_001103219.1; NP_766592.2. C-RRADGKPIARK) |
| Conjugation | Un-conjugated |
| Alternate Names | Brain-derived immunoglobulin superfamily protein 2; AXCAM; BIG-2; Contactin-4 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 150 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CNTN4 |
| Gene Full Name | contactin 4 |
| Background | This gene encodes a member of the contactin family of immunoglobulins. Contactins are axon-associated cell adhesion molecules that function in neuronal network formation and plasticity. The encoded protein is a glycosylphosphatidylinositol-anchored neuronal membrane protein that may play a role in the formation of axon connections in the developing nervous system. Deletion or mutation of this gene may play a role in 3p deletion syndrome and autism spectrum disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011] |
| Function | Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis. [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. Secreted. [UniProt] |
| Calculated MW | 113 kDa |
Images (2) Click the Picture to Zoom In
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ARG59753 anti-CNTN4 antibody WB image
Western blot: 35 µg of Mouse olfactory bulb (Lane 1 and 3) and cerebral cortex (Lane 2 and 4), comparing wild type (Lane 3 and 4) with KO Mouse (Lane 1 and 2). The blots were stained with ARG59753 anti-CNTN4 antibody at 0.5 µg/ml dilution and incubated for 1 hour.
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ARG59753 anti-CNTN4 antibody WB image
Western blot: HEK293 overexpressing Mouse CNTN4. 35 µg of cell lysate (in RIPA buffer) stained with ARG59753 anti-CNTN4 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
