ARG11063
anti-CNPase antibody [1H10]
anti-CNPase antibody [1H10] for IHC-Frozen sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [1H10] recognizes CNPase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1H10 |
| Isotype | IgG1 |
| Target Name | CNPase |
| Antigen Species | Human |
| Immunogen | Full length Human recombinant CNPase protein expressed in and purified from E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | CNPase; 2',3'-cyclic-nucleotide 3'-phosphodiesterase; CNP1; CNP; EC 3.1.4.37 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 46, 48 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS, 5mM Sodium azide and 50% Glycerol. |
| Preservative | 5mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CNP |
| Gene Full Name | 2',3'-cyclic nucleotide 3' phosphodiesterase |
| Function | May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. [UniProt] |
| Calculated MW | 48 kDa |
Images (2) Click the Picture to Zoom In
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ARG11063 anti-CNPase antibody [1H10] IHC-Fr image
Immunohistochemistry: Rat cerebellum section stained with ARG11063 anti-CNPase antibody [1H10] (green) at 1:500 dilution and costained with chicken pAb to NF-M (red) at 1:1000 dilution. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.
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ARG11063 anti-CNPase antibody [1H10] WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain and Mouse spinal cord lysates stained with ARG11063 anti-CNPase antibody [1H10] at 1:2000 dilution.
A doublet of bands at 46 and 48 kDa correspond to the major isotypes of the CNP protein.
