ARG42498
anti-CLTC / Clathrin heavy chain antibody
anti-CLTC / Clathrin heavy chain antibody for ICC/IF,Western blot and Human,Mouse,Rat,Dog,Monkey
Overview
| Product Description | Goat Polyclonal antibody recognizes CLTC / Clathrin heavy chain |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dog, Mk |
| Tested Application | ICC/IF, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CLTC / Clathrin heavy chain |
| Antigen Species | Human |
| Immunogen | Purified recombinant peptide within aa. 50 to the N-terminus of Human CLTC / Clathrin heavy chain. |
| Conjugation | Un-conjugated |
| Alternate Names | CHC17; CHC; Clathrin heavy chain 1; Hc; CLH-17; CLTCL2; Clathrin heavy chain on chromosome 17 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | AtT-20 | ||||||
| Observed Size | ~ 185 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.05% Sodium azide and 20% Glycerol. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 20% Glycerol |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CLTC |
| Gene Full Name | clathrin, heavy chain (Hc) |
| Background | Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008] |
| Function | Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:16968737, PubMed:21297582). The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension (PubMed:23532825). Plays a role in early autophagosome formation (PubMed:20639872). [UniProt] |
| Cellular Localization | Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Melanosome. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles. [UniProt] |
| Calculated MW | 192 kDa |
Images (2) Click the Picture to Zoom In
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ARG42498 anti-CLTC / Clathrin heavy chain antibody ICC/IF image
Immunofluorescence: Hepa1-6 cells were fixed with methanol. Cells were stained with ARG42498 anti-CLTC / Clathrin heavy chain antibody (green) at 1:50 dilution. Nuclear staining (blue).
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ARG42498 anti-CLTC / Clathrin heavy chain antibody WB image
Western blot: 100 µg of AtT-20 cell lysate stained with ARG42498 anti-CLTC / Clathrin heavy chain antibody at 1:500 dilution.
