ARG45675

anti-CLTB antibody

anti-CLTB antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CLTB
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CLTB
Antigen Species Human
Immunogen Synthetic peptide corresponding to middle region of human CLTB.
Conjugation Un-conjugated
Alternate Names CLTB; Clathrin Light Chain B; Lcb; Clathrin, Light Polypeptide (Lcb); Clathrin, Light Chain (Lcb); Clathrin, Light Chain B

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 32 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 116561 Rat CLTB

GeneID: 1212 Human CLTB

GeneID: 74325 Mouse CLTB

Gene Symbol CLTB
Gene Full Name Clathrin Light Chain B
Background Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Function Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. [UniProt]
Cellular Localization Coated pit; Cytoplasmic vesicle; Membrane. [UniProt]
Calculated MW 25 kDa
PTM Acetylation; Disulfide bond; Phosphoprotein. [UniProt]

Images (7) Click the Picture to Zoom In

  • ARG45675 anti-CLTB antibody IHC-P image

    Immunohistochemistry: Human endometrial cancer stained with ARG45675 anti-CLTB antibody at 2 μg/ml dilution.

  • ARG45675 anti-CLTB antibody WB image

    Western blot: PC-3 stained with ARG45675 anti-CLTB antibody at 0.5 μg/ml dilution.

  • ARG45675 anti-CLTB antibody FACS image

    Flow Cytometry: A431 stained with ARG45675 anti-CLTB antibody at 1 µg/10^6 cells dilution.

  • ARG45675 anti-CLTB antibody IHC-P image

    Immunohistochemistry: Rat cerebellum stained with ARG45675 anti-CLTB antibody at 2 μg/ml dilution.

  • ARG45675 anti-CLTB antibody WB image

    Western blot: Rat thymus stained with ARG45675 anti-CLTB antibody at 0.5 μg/ml dilution.

  • ARG45675 anti-CLTB antibody IHC-P image

    Immunohistochemistry: Mouse cerebellum stained with ARG45675 anti-CLTB antibody at 2 μg/ml dilution.

  • ARG45675 anti-CLTB antibody WB image

    Western blot: Mouse thymus stained with ARG45675 anti-CLTB antibody at 0.5 μg/ml dilution.