ARG65434
anti-CLIC5a antibody [CLIC5-02]
anti-CLIC5a antibody [CLIC5-02] for Flow cytometry,Western blot and Human
Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [CLIC5-02] recognizes CLIC5a |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Specificity | The clone CLIC5-02 recognizes CLIC5a, a 32 kDa protein which is component of actin complexes. Crossreactivity with CLIC5b was not determined. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CLIC5-02 |
| Isotype | IgG2b |
| Target Name | CLIC5a |
| Antigen Species | Human |
| Immunogen | KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a |
| Conjugation | Un-conjugated |
| Alternate Names | Chloride intracellular channel protein 5; DFNB102; DFNB103; MST130; MSTP130 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NZA1 Human Chloride intracellular channel protein 5 |
|---|---|
| Gene Symbol | CLIC5 |
| Gene Full Name | chloride intracellular channel 5 |
| Background | CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures. |
| Function | Required for normal hearing (PubMed:24781754). It is necessary for the formation of stereocilia in the inner ear and normal development of the organ of Corti (By similarity). Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture (PubMed:15184393, PubMed:18028448, PubMed:20335315). [UniProt] |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 47 kDa |
Images (1) Click the Picture to Zoom In
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ARG65434 anti-CLIC5a antibody [CLIC5-02] WB image
Western blot: A) CLIC5a-transfected HEK293 cell lysate, B) untransfected HEK293 cell lysate stained with ARG65434 anti-CLIC5a antibody [CLIC5-02].
