ARG42357
anti-CLEC9A antibody [8F9] (PE)
anti-CLEC9A antibody [8F9] (PE) for Flow cytometry and Human
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [8F9] recognizes CLEC9A |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 8F9 |
| Isotype | IgG2a |
| Target Name | CLEC9A |
| Antigen Species | Human |
| Immunogen | RBL-2H3 cells expressing Human CLEC9A fused to an HA epitope. |
| Conjugation | PE |
| Alternate Names | UNQ9341; C-type lectin domain family 9 member A; CD370; DNGR-1; DNGR1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6UXN8 Human C-type lectin domain family 9 member A |
|---|---|
| Gene Symbol | CLEC9A |
| Gene Full Name | C-type lectin domain family 9, member A |
| Background | CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells (Huysamen et al., 2008 [PubMed 18408006]).[supplied by OMIM, Aug 2008] |
| Function | Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner. [UniProt] |
| Cellular Localization | Membrane; Single-pass type II membrane protein. [UniProt] |
| Calculated MW | 27 kDa |
| PTM | N-glycosylated. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG42357 anti-CLEC9A antibody [8F9] (PE) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG42357 anti-CLEC9A antibody [8F9] (PE) at 10 µl / 100 µl of peripheral whole blood.
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ARG42357 anti-CLEC9A antibody [8F9] (PE) FACS image
Flow Cytometry: Human peripheral blood mononuclear cells stained with ARG42357 anti-CLEC9A antibody [8F9] (PE) at 10 µl / 100 µl of peripheral whole blood and ARG53938 anti-HLA DR antibody [MEM-12] (APC) at 10 µl / 100 µl of peripheral whole blood.
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ARG42357 anti-CLEC9A antibody [8F9] (PE) FACS image
Flow Cytometry: Separation of Human CD370 positive HLA-DR positive cells (red-filled) from CD370 negative HLA-DR negative cells (black-dashed). Human peripheral whole blood stained with ARG42357 anti-CLEC9A antibody [8F9] (PE) at 10 µl / 100 µl of peripheral whole blood.
