ARG43641
anti-CLEC2 / CLEC1B antibody
anti-CLEC2 / CLEC1B antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CLEC2 / CLEC1B |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody can block the interaction of human CLEC-2 with its endogenous ligand Podoplanin. This antibody does not cross react with Rabbit CLEC-2 when tested via FACS. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CLEC2 / CLEC1B |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within a.a. 40-80 of Human CLEC2. |
| Conjugation | Un-conjugated |
| Protein Full Name | C-type lectin domain family 1 member B |
| Alternate Names | CLEC2; C-type lectin domain family 1 member B; CLEC2B; PRO1384; C-type lectin-like receptor 2; CLEC-2; 1810061I13Rik; QDED721 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 28 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | Batch dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9P126 Human C-type lectin domain family 1 member B |
|---|---|
| Gene Symbol | CLEC1B |
| Gene Full Name | C-type lectin domain family 1, member B |
| Background | Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 (KLRD1; MIM 602894) and NKG2D (KLRC4; MIM 602893), that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 is a C-type lectin-like receptor expressed in myeloid cells and NK cells (Colonna et al., 2000 [PubMed 10671229]).[supplied by OMIM, Jan 2011] |
| Function | C-type lectin-like receptor that functions as a platelet receptor for the lymphatic endothelial marker, PDPN (PubMed:18215137). After ligand activation, signals via sequential activation of SRC and SYK tyrosine kinases leading to activation of PLCG2 (PubMed:18955485). (Microbial infection) Acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (SYK) and initiation of downstream tyrosine phosphorylation events and activation of PLCG2 (PubMed:16174766, PubMed:18955485). (Microbial infection) Acts as an attachment factor for Human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets (PubMed:16940507). [UniProt] |
| Cellular Localization | Membrane |
| Calculated MW | 27 kDa |
| PTM | Glycosylated. Phosphorylated on tyrosine residue in response to rhodocytin binding. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG43641 anti-CLEC2 / CLEC1B antibody WB image
Western blot: Human testis lysates were stained with ARG43641 anti-CLEC2 / CLEC1B antibody at 1:2000 dilution, overnight at 4°C.
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ARG43641 anti-CLEC2 / CLEC1B antibody WB image
Western blot: HepG2 cells lysates were stained with ARG43641 anti-CLEC2 / CLEC1B antibody at 1:2000 dilution, overnight at 4°C.
